Related Experiment Video
Updated: Jul 1, 2025
09:16
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
Published on: September 1, 2019
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RN7SL1 may be translated under oncogenic conditions
Tomoaki Hara1, Sikun Meng1, Yoshiko Tsuji1
1Department of Medical Data Science, Center of Medical Innovation and Translational Research, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Summary
The RNA component of signal recognition particle 7SL1 (RN7SL1) small open reading frame (smORF) may be translated into peptides. GFP-positive cells expressing RN7SL1-GFP exhibited an oncogenic state.
Area of Science:
- Molecular Biology
- RNA Biology
- Cancer Biology
Background:
- The RNA component of signal recognition particle 7SL1 (RN7SL1) is a non-coding RNA.
- RN7SL1 contains a small open reading frame (smORF) of unknown translational capacity.
Purpose of the Study:
- To investigate the potential translation of the RN7SL1 smORF.
- To explore the cellular consequences of RN7SL1 smORF expression.
Main Methods:
- Constructed a fusion gene replacing the RN7SL1 smORF with Green Fluorescent Protein (GFP).
- Transfected 293T cells with the RN7SL1-GFP construct.
- Analyzed GFP-positive cells using RNA sequencing (RNA-seq).
Main Results:
- Observed GFP fluorescence in transfected cells, indicating expression of the fusion protein.
- RNA-seq analysis revealed that GFP-positive cells were in an oncogenic state.
- These findings suggest RN7SL1 smORF translation under specific conditions.
Conclusions:
- The study provides evidence that the RN7SL1 smORF can be translated into peptides.
- RN7SL1 smORF translation is associated with an oncogenic cellular state.
- Further research is warranted to elucidate the mechanisms and implications of RN7SL1 translation.
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