Related Experiment Video
Updated: Jul 1, 2025

Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
Retron Library Recombineering: Next Powerful Tool for Genome Editing after CRISPR/Cas
Navdeep Kaur1, Pratap Kumar Pati1
1Department of Biotechnology, Guru Nanak Dev University, Amritsar 143005, Punjab, India.
Abstract:
Retron library recombineering (RLR) is a powerful tool in the field of genome editing that exceeds the scope and specificity of the CRISPR/Cas technique. In RLR, single-stranded DNA produced in vivo by harnessing the in-built potential of bacterial retrons is used for replication-dependent genome editing. RLR introduces several genomic variations at once, resulting in pooled and barcoded variant libraries, thus permitting multiplexed applications. Retron-generated RT-DNA has already shown promise for use in genome editing. Thus, this new tool will result in fresh, intriguing, and surprising developments in molecular biology and its juncture with other disciplines of research, including medicine, agriculture, and microbiology. In this review, we discuss the current state of this brand-new tool that could eventually boost genome editing research.
Related Concept Videos
Homologous Recombination
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
CRISPR
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

