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Cells and Secretions of the Pancreas

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The pancreas, a vital organ within the abdominal cavity, plays dual roles in the digestive and endocrine systems, collaborating with exocrine and endocrine cells to maintain optimal digestion and blood sugar levels.
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High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

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Published on: January 23, 2018

KSRP improves pancreatic beta cell function and survival.

Leticia Barssotti¹, Gabriela Moreira Soares¹, Emílio Marconato-Júnior¹

¹Obesity and Comorbidities Research Center (OCRC), Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083864, Brazil.

Scientific Reports

|March 14, 2024

Summary

KH-type splicing regulatory protein (KSRP) is crucial for pancreatic beta cell health. Reduced KSRP levels worsen beta cell dysfunction and insulin secretion, suggesting KSRP as a therapeutic target for type 2 diabetes.

Keywords:

Cell death ER stress Insulin secretion KSRP Pancreatic beta cell

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Area of Science:

Cell Biology
Endocrinology
Molecular Biology

Background:

Pancreatic beta cell dysfunction underlies type 2 diabetes (T2D).
Identifying novel proteins regulating beta cell stress response is critical for therapeutic development.
KH-type splicing regulatory protein (KSRP) impacts gene expression but its role in beta cells is unknown.

Purpose of the Study:

To investigate the role of KSRP in pancreatic beta cell function and survival under stress.
To determine if KSRP expression is altered in models of beta cell dysfunction.

Main Methods:

Assessed KSRP expression in pancreatic islets from diet-induced obese mice.
Utilized INS-1E cells exposed to stressors (palmitate, CPA) to model beta cell dysfunction.
Performed KSRP knockdown and overexpression experiments in beta cells.

Main Results:

KSRP expression was reduced in all tested beta cell dysfunction models.
KSRP knockdown led to increased beta cell death and impaired insulin secretion.
KSRP overexpression protected beta cells from death and enhanced insulin secretion.

Conclusions:

KSRP plays a protective role in pancreatic beta cells.
KSRP is a potential therapeutic target for preserving beta cell function and preventing type 2 diabetes.