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Genomic DNA in Eukaryotes

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The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Bees.

Thomas James Wood1, Hugo Gaspar2, Romain Le Divelec3

  • 1Naturalis Biodiversity Center, Leiden, Netherlands Naturalis Biodiversity Center Leiden Netherlands.

Biodiversity Data Journal
|March 14, 2024
PubMed
Summary

This study established a DNA barcode reference collection for Iberian bees, adding 75 new species and 112 BINs to global databases. The findings highlight genetic diversity and potential new species, aiding taxonomic revisions.

Keywords:
DNA barcodeHymenopteracytochrome c oxidase subunit I (COI)occurrence recordspeninsular Portugalpeninsular Spainpollinatorspecies distributions

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Area of Science:

  • Ecology
  • Genetics
  • Biodiversity

Background:

  • Bees are crucial pollinators in terrestrial ecosystems.
  • The Iberian Peninsula hosts approximately 1,100 bee species, with nearly 100 endemic.
  • A significant portion of Iberian bee diversity remains understudied.

Purpose of the Study:

  • To construct a DNA barcode reference collection for Iberian bees.
  • To document bee species diversity and genetic variation in the Iberian Peninsula.
  • To provide a baseline for taxonomic revisions and biodiversity assessments.

Main Methods:

  • Morphological identification of bee specimens.
  • DNA barcoding of 1,059 bee specimens representing 514 Iberian species.
  • Data compilation into the InBIO Barcoding Initiative Database.

Main Results:

  • Sequencing covered 47% of Iberian bee species and 21% of endemic species.
  • 75 species and 112 BINs are new additions to the Barcode of Life Data System (BOLD).
  • Multiple BINs assigned to single species suggest unrecognized diversity, necessitating integrative taxonomy.

Conclusions:

  • The DNA barcode data significantly enhances understanding of Iberian bee genetic diversity.
  • This dataset serves as a vital baseline for ongoing taxonomic research in the West Palaearctic.
  • The findings underscore the importance of DNA barcoding for biodiversity monitoring and conservation.