Evaluation of Antioxidant, Anti-inflammatory, and Antimicrobial Activities of Raspberry Fruit Extract: An In Vitro...

Area of Science:

• Phytochemistry
• Pharmacology
• Natural Products

Background:

• Rubus idaeus (raspberry) is rich in bioactive compounds with nutraceutical properties.
• Raspberry consumption is linked to reduced risk of chronic diseases like diabetes, cancer, and heart disease.

Purpose of the Study:

• To investigate the antimicrobial, antioxidant, and anti-inflammatory effects of an aqueous raspberry extract.
• To evaluate the potential of raspberry extract as a natural therapeutic agent.

Main Methods:

• Aqueous raspberry extract was prepared from Rubus idaeus.
• Antimicrobial activity tested against Streptococcus mutans and Candida albicans using agar-well diffusion.
• Antioxidant activity assessed via DPPH and hydrogen peroxide radical scavenging assays.
• Anti-inflammatory activity evaluated using bovine serum albumin and egg albumin denaturation assays.

Main Results:

• Raspberry extract showed strong antimicrobial activity against S. mutans (26 mm zone) and C. albicans (24 mm zone) at 100 µL.
• Extract exhibited significant antioxidant activity: 87.42% hydrogen peroxide scavenging and 91.12% DPPH radical inhibition at 50 µg/mL.
• Effective anti-inflammatory activity observed, inhibiting BSA denaturation by 80% and egg albumin by 77% at 50 μg/mL.

Conclusions:

• Aqueous raspberry extract possesses notable antimicrobial, antioxidant, and anti-inflammatory properties.
• The extract shows promise as a natural alternative in oral medicine for conditions like oral mucosal lesions, candidiasis, and oral cancer.
• Further in vivo studies and clinical trials are recommended to validate therapeutic potential.