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Updated: Jul 1, 2025

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Nonpathogenic leaf-colonizing bacteria elicit pathogen-like responses in a colonization density-dependent manner.

Moritz Miebach^1,2, Léa Faivre³, Daniel Schubert³

¹School of Biological Sciences University of Canterbury Christchurch New Zealand.

Plant-Environment Interactions (Hoboken, N.J.)

|March 14, 2024

Summary

This summary is machine-generated.

Plant leaves respond slowly to bacterial colonization, with responses differing in strength, not type. High densities of nonpathogenic bacteria trigger pathogen-like defense mechanisms in Arabidopsis thaliana.

Keywords:

Arabidopsis Pseudomonas Williamsia chromatin state analysis microbiota phyllosphere transcriptomics

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Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Published on: October 6, 2022

Area of Science:

Plant-microbe interactions
Microbial ecology
Molecular plant pathology

Background:

The leaf microbiota plays a crucial role in plant health, yet its interactions with host plants remain poorly understood.
Understanding these interactions is vital for agriculture and plant science.

Purpose of the Study:

To investigate the transcriptomic and epigenetic responses of *Arabidopsis thaliana* to bacterial leaf colonization.
To determine the timing and nature of plant defense responses to both pathogenic and nonpathogenic bacteria.

Main Methods:

Axenically grown *Arabidopsis thaliana* plants were spray-inoculated with diverse bacteria.
Whole-transcriptome sequencing was employed to track gene expression changes over time.
In silico epigenetic analysis was performed to complement transcriptomic data.

Main Results:

Significant transcriptomic changes, particularly in ethylene marker (ARL2) expression, were observed 2-4 days post-inoculation.
Leaf transcriptional responses to pathogenic and nonpathogenic bacteria differed in magnitude but not in type.
High bacterial titers of nonpathogenic *Williamsia* sp. Leaf354 induced disease phenotypes and strong plant defense responses.

Conclusions:

Plant responses to spray-inoculated bacteria are not rapid, with significant changes occurring after 2-4 days.
The type of transcriptional response is conserved, regardless of bacterial pathogenicity, with strength varying based on bacterial load.
Plants mount pathogen-like defense responses even to high titers of nonpathogenic bacteria, indicating a sensitive detection system.