Multiple interspecies recombination events documented by whole-genome sequencing in multidrug-resistant Haemophilus

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Updated: Jul 1, 2025

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

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Charlotte Michel1,2, Maria De Los Angeles Argudín3, Magali Wautier1,2

  • 1Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles (LHUB-ULB), Rue Haute 322, 1000 Brussels, Belgium.

Access Microbiology
|March 14, 2024
PubMed
Abstract

Insights

Increasing antibiotic resistance in Haemophilus influenzae (Hi) is a growing concern. Whole-genome sequencing revealed multi-drug resistant Hi isolates with complex resistance mechanisms, highlighting the need for global monitoring.

Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Haemophilus influenzae (Hi) is increasingly exhibiting resistance to critical antibiotics.
  • This study focuses on non-typeable Hi isolates with unusual multi-resistant profiles.

Purpose of the Study:

  • To analyze the whole-genome sequences of three multi-drug resistant non-typeable Hi isolates.
  • To investigate the genetic basis of antimicrobial resistance (AMR) in these isolates.
  • To understand the epidemiological context of these resistant Hi strains.

Main Methods:

  • Whole-genome sequencing (WGS) of three non-typeable Hi isolates.
  • Analysis of homologous recombination in key genes (ftsI, murE, murF).
  • Antimicrobial resistance (AMR) genotyping and core-genome multilocus sequence typing (MLST).

Main Results:

  • Isolates showed interspecies recombination patterns and were multidrug resistant.
  • Resistance to amoxicillin-clavulanic acid and reduced susceptibility to meropenem were observed.
  • WGS identified mobile genetic elements, fluoroquinolone resistance mutations, and ompP2 remodelling.

Conclusions:

  • Hi's evolution towards AMR is complex, involving recombination, horizontal gene transfer, and mutations.
  • Further analysis of Hi communities and global monitoring of non-typeable strains are crucial.
  • Understanding these mechanisms is key to combating Hi AMR.
Keywords:
DNA uptake machineryHaemophilus influenzaemultidrug resistantrecombination eventsuptake signal sequencewhole-genome sequencing

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