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Related Experiment Videos

Transferrin in isolated cells from rat duodenum and jejunum.

K Osterloh, K Schümann, C Ehtechami

    Blut
    |July 1, 1985
    PubMed
    Summary

    Iron deficiency increases mucosal transferrin-like immunoreactivity (TLIR) in rat intestines. Washing isolated intestinal cells significantly reduces TLIR, suggesting it is primarily extracellular.

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    Abnormal body iron distribution and erythropoiesis in a novel mouse model with inducible gain of iron regulatory protein (IRP)-1 function.

    Journal of molecular medicine (Berlin, Germany)·2013

    Area of Science:

    • Gastroenterology
    • Iron Metabolism
    • Cell Biology

    Background:

    • Transferrin is crucial for iron transport.
    • Mucosal transferrin's role in iron absorption is not fully understood.
    • Iron deficiency impacts intestinal physiology.

    Purpose of the Study:

    • To quantify transferrin-like immunoreactivity (TLIR) in rat intestinal mucosa.
    • To investigate the effect of iron deficiency on mucosal TLIR.
    • To determine the cellular localization of TLIR.

    Main Methods:

    • Used a 2-site immunoradiometric assay (IRMA) to measure TLIR.
    • Examined scraped mucosal tissue and isolated cells from rat duodenum and jejunum.
    • Assessed the impact of a washing procedure on TLIR in isolated cells.

    Main Results:

    • Iron-deficient rats showed approximately double the TLIR in scraped mucosal tissue compared to normal rats.
    • Isolated intestinal cells from both normal and iron-deficient rats contained substantial TLIR (320-510 ng/mg dry weight).
    • Washing isolated cells led to a nearly tenfold decrease in TLIR, while RNA content remained stable.

    Conclusions:

    • Mucosal TLIR is elevated in iron deficiency.
    • A significant portion of mucosal TLIR is associated with the extracellular compartment of intestinal cells.
    • TLIR is not tightly bound to intestinal cells and can be removed by washing.

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