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Updated: Jun 30, 2025

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HPV16 E7 modulates the cell surface expression of MET and CD109 via the AP2 complex

Oscar Trejo-Cerro1, Om Basukala2, Michael P Myers1

  • 1International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34149, Trieste, Italy.

Tumour Virus Research
|March 14, 2024
PubMed

Insights

Human papillomavirus (HPV) oncoprotein E7 affects cell surface protein levels by interacting with the AP2 complex. HPV-16 E7 dysregulates MET and CD109 expression, impacting cellular trafficking pathways.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Virology

Background:

  • Human papillomavirus (HPV) oncoproteins E6 and E7 are key factors in cervical cancer development.
  • These oncoproteins disrupt cellular pathways, including endocytic and cellular trafficking.
  • HPV-16 E7 specifically targets adaptor protein (AP) complexes involved in endocytosis.

Purpose of the Study:

  • To investigate the role of HPV-16 E7 in regulating cell surface protein expression.
  • To determine how HPV-16 E7 interacts with the AP2 complex.
  • To elucidate the impact of HPV-16 E7 on MET and CD109 membrane protein expression.

Main Methods:

  • Analysis of cell surface protein expression in NIKS cells engineered to express HPV-16 E7.
  • Investigating the interaction between HPV-16 E7, AP2, MET, and CD109 using cellular assays.
  • Comparing protein expression in HPV-positive cell lines with and without HPV-16 E7 expression.

Main Results:

  • HPV-16 E7 regulates cell surface proteins through interaction with the AP2 complex.
  • MET and CD109 membrane protein expression is upregulated in cells expressing HPV-16 E7.
  • HPV-16 E7 disrupts the interaction between MET, CD109, and AP2 proteins.
  • Absence of HPV-16 E7 leads to downregulation of MET and CD109 in HPV-positive cell lines.

Conclusions:

  • HPV-16 E7 significantly impacts cell surface protein expression and cellular trafficking.
  • The interaction between HPV-16 E7 and AP2 influences the regulation of MET and CD109.
  • These findings expand the understanding of HPV oncoprotein functions and identify potential therapeutic targets.
Keywords:
AP2 complexCD109HPV E7MET

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