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Related Experiment Video

Updated: Jun 30, 2025

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Single-cell tracking as a tool for studying EMT-phenotypes.

Ellen Marie Botne Quinsgaard1, Mónica Suárez Korsnes2, Reinert Korsnes3

  • 1Norwegian University of Science and Technology (NTNU), Department of Clinical and Molecular Medicine, NO-7491 Trondheim, Norway.

Experimental Cell Research
|March 14, 2024
PubMed
Summary

Label-free single-cell video tracking offers a powerful method for studying Epithelial-Mesenchymal Transition (EMT), revealing cell behavior variations and trait heritability in cancer metastasis research.

Keywords:
Cell migrationEpithelial-mesenchymal transitionInheritanceMorphologySingle-cell trackingViability

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Area of Science:

  • Cell Biology
  • Cancer Research
  • Biotechnology

Background:

  • Epithelial-Mesenchymal Transition (EMT) is a complex cellular process crucial for development and disease, particularly cancer metastasis.
  • Understanding EMT heterogeneity is vital for developing effective cancer therapies.
  • Current methods for studying EMT face challenges due to context-dependent biomarker roles.

Purpose of the Study:

  • To demonstrate the utility of label-free single-cell video tracking for comprehensive in vitro analysis of EMT.
  • To quantify single-cell behaviors and morphological changes during induced EMT.
  • To investigate the heritability of cellular traits in sister cells undergoing EMT.

Main Methods:

  • Performed 72-hour label-free single-cell video tracking on MDA-MB-468 cells undergoing induced EMT.
  • Quantified single-cell migration speed, morphology, and behavioral patterns.
  • Analyzed cell death, proliferation, and mitotic events, including sister-cell re-fusion.

Main Results:

  • Observed significant variations in migration speed and dose-response patterns during EMT.
  • Identified preferred paths for morphological transitions and a correlation between speed and morphology.
  • Documented increased cell death, reduced proliferation, mitotic failures, and sister-cell re-fusion.

Conclusions:

  • Label-free single-cell video tracking provides a robust approach for detailed EMT phenotyping.
  • The study highlights the heterogeneity of EMT at the single-cell level and the heritability of traits like speed and morphology.
  • Findings contribute to a deeper understanding of EMT in cancer metastasis and potential therapeutic targets.