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Enhancing a multi-purpose artificial urine for culture and gene expression studies of uropathogenic Escherichia coli
Patricia T Rimbi1, Nicky O'Boyle2,3, Gillian R Douce1
1School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, United Kingdom.
Aims:
The main objective of this study was to modify a recently reported multi-purpose artificial urine (MP-AU) for culture and gene expression studies of uropathogenic Escherichia coli (UPEC) strains.
Methods And Results:
We used liquid chromatography mass spectrometry (LC-MS) to identify and adjust the metabolic profile of MP-AU closer to that of pooled human urine (PHU). Modification in this way facilitated growth of UPEC strains with growth rates similar to those obtained in PHU. Transcriptomic analysis of UPEC strains cultured in enhanced artificial urine (enhanced AU) and PHU showed that the gene expression profiles are similar, with <7% of genes differentially expressed between the two conditions.
Conclusions:
Enhancing an MP-AU with metabolites identified in PHU allows the enhanced AU to be used as a substitute for the culture and in vitro gene expression studies of UPEC strains.
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