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Draft Genome Sequence of Pantoea sp. Strain MHSD4, a Bacterial Endophyte With Bioremediation Potential

Dimpho Michelle Morobane1, Khuthadzo Tshishonga1, Mahloro Hope Serepa-Dlamini1

  • 1Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Doornfontein Campus, Doornfontein, Johannesburg, South Africa.

Evolutionary Bioinformatics Online
|March 15, 2024
PubMed

Insights

The genome of Pantoea sp. strain MHSD4, a bacterial endophyte from Pellaea calomelanos, was sequenced. This bacterium shows potential for bioremediation due to identified genes for heavy metal tolerance and pollutant degradation.

Area of Science:

  • Microbiology
  • Genomics
  • Biotechnology

Background:

  • Pantoea sp. strain MHSD4 is a bacterial endophyte isolated from the medicinal plant Pellaea calomelanos.
  • Endophytic bacteria offer novel avenues for biotechnological applications.

Purpose of the Study:

  • To report the draft whole genome sequence and annotation of Pantoea sp. strain MHSD4.
  • To identify genes related to bioremediation and heavy metal tolerance.

Main Methods:

  • Whole genome sequencing of Pantoea sp. strain MHSD4.
  • Annotation using the National Center for Biotechnology Information Prokaryotic Genome Annotation Pipeline.

Main Results:

  • The draft genome size is 4,647,677 bp with a G+C content of 54.2% and 41 contigs.
  • 4395 genes were predicted, including 4235 protein-coding genes and 70 tRNAs.
  • Identified pathways for naphthalene and anthracene degradation, and genes for copper homeostasis and tolerance (e.g., copA, copD, cueO).

Conclusions:

  • Pantoea sp. strain MHSD4 possesses genes for bioremediation of polycyclic aromatic hydrocarbons and heavy metals.
  • The identified genes suggest significant biotechnological potential for environmental remediation applications.
Keywords:
Pantoeabacterial endophytebioremediationwhole genome sequencing

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