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Dual-Emissive Iridium(III) Complexes and Their Applications in Biological Sensing and Imaging
Zhipeng Ruan1, Jun Yang1, Yonghua Li1
1State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing, 210023, P. R. China.
Chembiochem : a European Journal of Chemical Biology
|March 15, 2024
Summary
Dual-emissive iridium(III) complexes offer advanced biological sensing and imaging. Their unique emission profiles enable self-calibrating quantification and orthogonal detection of multiple analytes.
Area of Science:
- Coordination Chemistry
- Photophysics
- Bioanalytical Chemistry
Background:
- Phosphorescent iridium(III) complexes exhibit unique excited triplet state properties.
- Typically, these complexes show single phosphorescence after efficient intersystem crossing (ISC) and internal conversion (IC).
- Restricted excited-state processes can lead to the less common dual emission phenomenon.
Purpose of the Study:
- To explore dual emission in iridium(III) complexes for enhanced biological sensing and imaging.
- To leverage dual emission for self-calibrating quantification and orthogonal multi-analyte detection.
- To focus on iridium(III) complexes exhibiting fluorescence-phosphorescence or phosphorescence-phosphorescence dual emission.
Main Methods:
- Synthesis and characterization of iridium(III) complexes.
- Photophysical studies to investigate excited-state dynamics and emission properties.
- Application development for biological sensing and imaging using dual-emissive probes.
Main Results:
- Demonstration of dual emission (fluorescence-phosphorescence or phosphorescence-phosphorescence) in iridium(III) complexes.
- Development of probes utilizing emission profile changes for quantification.
- Probes show self-calibration, unaffected by probe concentration or excitation power.
- Potential for orthogonal detection of multiple analytes at distinct wavelengths.
Conclusions:
- Dual-emissive iridium(III) complexes are promising for advanced biological probes.
- Emission profile response offers advantages over intensity-based sensing.
- These complexes enable sensitive, selective, and self-calibrated detection in biological systems.
