Quantitative mass spectrometry imaging (qMSI): A tutorial

Area of Science:

• Analytical Chemistry
• Biomedical Imaging
• Molecular Pathology

Background:

• Mass spectrometry imaging (MSI) is a powerful analytical technique for simultaneous detection of numerous chemical species within biological tissues, preserving spatial distribution.
• Current MSI applications excel at identifying and localizing molecules, significantly impacting health and disease research.
• The integration of quantitative mass spectrometry (qMS) with MSI is the next frontier for precise localization and quantification of disease markers or drug effects.

Purpose of the Study:

• To provide a tutorial on designing quantitative mass spectrometry imaging (qMSI) experiments.
• To highlight key considerations and methods for overcoming the complexities of qMSI.
• To assist researchers new to the field of MSI in adopting quantitative approaches.

Main Methods:

• Detailed discussion of critical factors in designing qMSI experiments.
• Explanation of established and emerging methodologies for quantitative analysis in MSI.
• Focus on practical aspects relevant to experimental setup and data interpretation.

Main Results:

• Provides a framework for successful qMSI experimental design.
• Identifies strategies to address challenges in quantitative molecular imaging.
• Empowers researchers to implement qMSI for advanced biomedical studies.

Conclusions:

• Quantitative mass spectrometry imaging (qMSI) represents a significant advancement in molecular imaging.
• Successful qMSI design and execution are crucial for accurate disease and drug action analysis.
• This tutorial serves as a foundational guide for researchers entering the field of qMSI.