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[Experimental protective action of kanamycin, ampicillin and their combination with methyluracil and pyrogenal]
Abstract:
Efficacy of kanamycin, ampicillin and their combinations with methyluracyl and pyrogenal in experimental Coli infections was studied. The antibiotics were administered an hour after the infection. Methyluracyl and pyrogenal were used according to 2 schemes. Scheme No. I: the drug is used daily for 7 days in increasing doses, the last dose is administered 24 hours before the infection. Scheme No. 2: the drug is used once at the moment of the infection. The methyluracyl doses were: 0.5, 1.0, 2.5 mg and 5 mg and 5 mg per a mouse during the following 4 days. The pyrrogenal doses were: 5, 10, 15, 25, 30 and 35 minimum pyrogenic doses. 5 mg of methyluracyl and 35 minimum pyrogenic doses of pyrogenal were used according to scheme No. 2. The most pronounced increase in the efficacy of kanamycin, ampicillin and their combination was observed in the animals treated simultaneously with methyluracyl and pyrogenal according to scheme No. 1. The efficacy of kanamycin and ampicillin increased 3 and 2.68 times respectively. ED50 of kanamycin and ampicillin used in combination in the animals treated with methyluracyl and pyrogenal was lowered 4 and 2.9 times respectively as compared to that in the animal groups treated only with the antibiotic combination and 21 and 15.2 times respectively when the antibiotics were used alone. Sanation of the animal organs was also rather successful. A single administration of methyluracyl and pyrogenal simultaneously with the infection (scheme No. 2) had a lower effect on the efficacy.
Insights
This study shows that combining kanamycin and ampicillin with methyluracil and pyrogenal significantly enhances their efficacy against experimental E. coli infections. Optimal results were achieved with a specific treatment regimen, improving antibiotic effectiveness.
Area of Science:
- Microbiology
- Pharmacology
- Immunology
Background:
- Antibiotic resistance in E. coli poses a significant challenge in treating infections.
- Exploring adjunct therapies to potentiate antibiotic efficacy is crucial for improving treatment outcomes.
Purpose of the Study:
- To evaluate the synergistic effects of methyluracil and pyrogenal with kanamycin and ampicillin in experimental E. coli infections.
- To determine the optimal administration scheme for these combined therapies.
Main Methods:
- Experimental E. coli infection model in animals.
- Administration of kanamycin and ampicillin alone and in combination with methyluracil and pyrogenal.
- Evaluation of two distinct treatment schemes for methyluracil and pyrogenal administration.
- Assessment of antibiotic efficacy through survival rates and organ sanation.
Main Results:
- Simultaneous administration of methyluracil and pyrogenal with kanamycin and ampicillin (Scheme 1) significantly enhanced antibiotic efficacy.
- Kanamycin and ampicillin efficacy increased 3-fold and 2.68-fold, respectively.
- The median effective dose (ED50) for the antibiotic combination was reduced by 4-fold and 2.9-fold when used with methyluracil and pyrogenal, compared to antibiotic combination alone.
Conclusions:
- Methyluracil and pyrogenal act as potentiators, significantly increasing the efficacy of kanamycin and ampicillin against E. coli.
- The timing and regimen of adjunct therapy administration are critical for maximizing therapeutic benefits.
- Combined therapy offers a promising strategy to combat experimental E. coli infections more effectively.
