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Related Experiment Video

Updated: Jun 30, 2025

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
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Data retrieval from archival renal biopsies using nonlinear microscopy.

Lucas C Cahill1,2, Tadayuki Yoshitake2, Milan Rosen3

  • 1Harvard-MIT Division of Health Sciences and Technology, Harvard Medical School and Massachusetts Institute of Technology, Cambridge, MA, United States of America.

Plos One
|March 15, 2024
PubMed
Summary
This summary is machine-generated.

Nonlinear microscopy (NLM) and optical clearing allow 3D imaging of renal biopsies from archival blocks. This technique enhances pathology examination and enables large-scale retrospective studies of kidney disease.

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Area of Science:

  • Nephrology
  • Biomedical Imaging
  • Pathology

Background:

  • Understanding renal disease relies on thorough renal biopsy examination.
  • Traditional methods involve microtome sectioning, which can be limiting.
  • Archival tissue blocks offer a valuable resource for retrospective studies.

Purpose of the Study:

  • To evaluate the utility of fluorescence nonlinear microscopy (NLM) combined with optical clearing for 3D visualization of renal biopsies.
  • To assess the feasibility of analyzing archival renal paraffin blocks using this technique.
  • To determine if NLM can provide quantitative insights into renal pathology.

Main Methods:

  • Archival renal paraffin blocks were deparaffinized and stained.
  • Optical clearing was performed using benzyl alcohol benzyl benzoate (BABB).
  • Nonlinear microscopy (NLM) was used for 3D imaging of entire biopsy fragments, with signals mapped to an H&E color scale.

Main Results:

  • Three-dimensional (3D) visualization of renal pathology was achieved without microtome sectioning.
  • Structures like cysts, glomeruli, exudative lesions, and Kimmelstiel-Wilson nodules were segmented in 3D.
  • Quantitative data on volume, diameter, and percent composition of pathological features were obtained, with a high glomerular count observed.

Conclusions:

  • Rapid optical clearing and NLM imaging enable more thorough examination of renal biopsies.
  • This technique is a promising tool for the analysis of archival paraffin blocks.
  • Archival blocks can serve as a significant resource for larger-scale retrospective studies in nephrology.