Related Experiment Video
Updated: Jun 30, 2025
09:17
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Published on: March 4, 2015
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KCTD10 regulates brain development by destabilizing brain disorder-associated protein KCTD13
Jianbo Cheng1, Zhen Wang1, Manpei Tang1
1Center for Medical Genetics, Hunan Key Laboratory of Medical Genetics, Key Lab of Rare Pediatric Diseases of Ministry of Education, School of Life Sciences, Central South University, Changsha, Hunan 410078, China.
Summary
Potassium channel tetramerization domain containing 10 (KCTD10) regulates neuronal progenitor development and brain size. KCTD10 deficiency causes motor deficits by increasing KCTD13 levels, impacting neurodevelopment.
Area of Science:
- Neuroscience
- Developmental Biology
- Molecular Biology
Background:
- The KCTD (potassium channel tetramerization domain) family is linked to neuropsychiatric disorders.
- The specific function of KCTD10 in brain development and disorders is largely unknown.
Purpose of the Study:
- To elucidate the physiological role of KCTD10 in brain development.
- To investigate the molecular mechanisms underlying KCTD10's function and its link to neurodevelopmental disorders.
Main Methods:
- Analysis of KCTD10 expression in developing brain.
- Generation and analysis of Kctd10-deficient mouse models.
- Identification and characterization of KCTD10-interacting proteins, including KCTD13.
- Assessment of KCTD13's role in neuronal progenitor cells.
- Evaluation of motor function in Kctd10 knockout mice.
Main Results:
- KCTD10 is highly expressed in neuronal progenitors and layer V neurons during brain development.
- Kctd10 deficiency leads to abnormal progenitor proliferation/differentiation, reduced deep-layer neurons, increased upper-layer neurons, and smaller brain size.
- KCTD10 interacts with KCTD13 and mediates its ubiquitination-dependent degradation.
- KCTD10 ablation increases KCTD13 levels, causing phenotypes similar to KCTD10 deficiency.
- Kctd10 knockout mice exhibit motor deficits.
Conclusions:
- KCTD10 plays a crucial role in regulating neuronal progenitor development and brain size.
- KCTD10's function is partly mediated through the degradation of KCTD13.
- This study provides insights into the pathogenesis of neurodevelopmental disorders involving KCTD10 and KCTD13.
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