Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Etiology and pathogens of fungal endophthalmitis].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2015
Same author

Eucommia ulmoides Oliv. bark aqueous extract inhibits osteoarthritis in a rat model of osteoarthritis.

Journal of ethnopharmacology·2015
Same author

Aucubin prevents interleukin-1 beta induced inflammation and cartilage matrix degradation via inhibition of NF-κB signaling pathway in rat articular chondrocytes.

International immunopharmacology·2015
Same author

Treatment with recombinant lubricin attenuates osteoarthritis by positive feedback loop between articular cartilage and subchondral bone in ovariectomized rats.

Bone·2015
Same author

Tet1-mediated DNA demethylation regulates neuronal cell death induced by oxidative stress.

Scientific reports·2015
Same author

Authors' reply.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2015

Related Experiment Video

Updated: Jun 30, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.2K

Polarization-resolved super-resolution second-harmonic generation imaging based on multifocal structured illumination

Yong Zhang, Chenshuang Zhang, Renlong Zhang

    Optics Letters
    |March 15, 2024
    PubMed
    Summary

    Researchers developed a new super-resolution microscopy technique combining multifocal structured illumination microscopy (MSIM) with polarization-resolved second-harmonic generation (PSHG) to reveal detailed collagen structures in tendons.

    More Related Videos

    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
    12:44

    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

    Published on: September 29, 2014

    20.0K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    8.9K

    Related Experiment Videos

    Last Updated: Jun 30, 2025

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.2K
    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
    12:44

    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

    Published on: September 29, 2014

    20.0K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    8.9K

    Area of Science:

    • Biophysics
    • Microscopy
    • Materials Science

    Background:

    • Polarization-resolved second-harmonic generation (PSHG) microscopy analyzes collagen structure.
    • Current PSHG resolution is limited by optical diffraction, averaging collagen fiber properties.
    • Super-resolution imaging is needed to resolve finer collagenous structures.

    Purpose of the Study:

    • To achieve polarization-resolved super-resolution imaging of second-harmonic generation (SHG) signals.
    • To overcome the diffraction limit in collagen structural analysis.
    • To investigate nanoscale collagen organization in biological tissues.

    Main Methods:

    • Combined multifocal structured illumination microscopy (MSIM) with PSHG.
    • Utilized MSIM to enhance the resolution of SHG imaging.
    • Applied the technique to analyze collagen fibrils in mouse tail tendons.

    Main Results:

    • Achieved polarization-resolved super-resolution imaging of SHG signals.
    • Observed periodic structures with an average pitch of 277 nm in mouse tail tendons.
    • Discovered a regular, alternating arrangement of fibril orientation within these periodic structures.

    Conclusions:

    • The developed MSIM-PSHG technique enables super-resolution imaging of collagen.
    • Revealed previously unobserved nanoscale periodic structures in tendon collagen.
    • Demonstrated a regular, alternating fibril orientation pattern within these structures, offering new insights into collagen organization.