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Updated: Jun 30, 2025
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
A reusable screen-printed carbon electrode-based aptasensor for the determination of chloramphenicol in food and
Krittapas Kaewnu1, Supatinee Kongkaew2, Sasimanas Unajak3
1Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Forensic Science Innovation and Service Center, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Abstract:
An electrochemical aptasensor was developed for the determination of chloramphenicol (CAP) in fresh foods and food products. The aptasensor was developed using Prussian blue (PB) and chitosan (CS) film. PB acts as a redox probe for detection and CS acts as a sorption material. The aptamer (Apt) was immobilized on a screen-printed carbon electrode (SPCE) modified with gold nanoparticles (AuNPs). Under optimum conditions, the linearity of the aptasensor was between 1.0 and 6.0 × 106 ng L-1 with a detection limit of 0.65 and a quantification limit of 2.15 ng L-1. The electrode could be regenerated up to 24 times without the use of chemicals. The aptasensor showed good repeatability (RSD <11.2%) and good reproducibility (RSD <7.7%). The proposed method successfully quantified CAP in milk, shrimp pond water and shrimp meat with good accuracy (recovery = 88.0 ± 0.6% to 100 ± 2%). The proposed aptasensor could be especially useful in agriculture to ensure the quality of food and the environment and could be used to determine other antibiotics.
