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  1. Home
  3. Porcine Transient Receptor Potential Channel 1 (trpc1) Regulates Muscle Growth Via The Wnt/β-catenin And Wnt/ca2+ Pathways.
  1. Home
  3. Porcine Transient Receptor Potential Channel 1 (trpc1) Regulates Muscle Growth Via The Wnt/β-catenin And Wnt/ca2+ Pathways.

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Porcine transient receptor potential channel 1 (TRPC1) regulates muscle growth via the Wnt/β-catenin and Wnt/Ca2+

Xin Hao1, Yu Fu1, Shixin Li1

  • 1State Key Laboratory of animal biotech breeding, Beijing Key Laboratory of animal genetic engineering, China Agricultural University, Beijing 100193, China.

International Journal of Biological Macromolecules
|March 15, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Porcine TRPC1 enhances skeletal muscle growth, repair, and endurance by increasing intracellular calcium and activating Wnt/β-catenin and Wnt/Ca2+ pathways. This process is promoted by Paired related homeobox 1 (Prrx1).

Keywords:
Muscle developmentTransient receptor potential channel 1Wnt pathway

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Area of Science:

  • Physiology
  • Molecular Biology
  • Muscle Biology

Background:

  • Transient receptor potential canonical (TRPC) channels regulate intracellular Ca2+, impacting physiological and pathological processes.
  • TRPC1's specific role in skeletal muscle development and growth requires further elucidation.

Purpose of the Study:

  • To investigate the effects of porcine TRPC1 (pTRPC1) on skeletal muscle growth and regeneration in transgenic mice.
  • To elucidate the molecular mechanisms underlying pTRPC1-mediated muscle development.

Main Methods:

  • Construction of transgenic mice expressing pTRPC1 (Tg-pTRPC1).
  • Assessment of muscle mass, fiber cross-sectional area, exercise endurance, and muscle repair.
  • Analysis of β-catenin expression, intracellular Ca2+ levels, and NFATC2/NFATC2IP complex nuclear translocation.
  • Investigation of the role of Paired related homeobox 1 (Prrx1) in regulating TRPC1 expression.

    • Main Results:

    • Tg-pTRPC1 mice exhibited increased muscle mass, fiber size, and exercise endurance.
    • pTRPC1 accelerated muscle repair and regeneration.
    • Overexpression of TRPC1 enhanced β-catenin expression and promoted myogenesis, partly reversible by β-catenin inhibitors.
    • TRPC1 facilitated Ca2+ accumulation and NFATC2/NFATC2IP nuclear translocation, activating Wnt/Ca2+ signaling.
    • Prrx1 was found to promote the expression of TRPC1, NFATC2, and NFATC2IP.

    Conclusions:

    • Porcine TRPC1 promotes skeletal muscle development and function.
    • TRPC1 activates both canonical Wnt/β-catenin and non-canonical Wnt/Ca2+ pathways for muscle growth.
    • Prrx1 plays a regulatory role in muscle development by influencing TRPC1 and associated signaling pathways.