Related Experiment Video
Updated: Jun 30, 2025
08:50
In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
Published on: April 18, 2025
391
Biomimetic hepatic lobules from three-dimensional imprinted cell sheets.
Jinglin Wang1, Danqing Huang1, Hanxu Chen2
1Department of Hepatobiliary Surgery, Hepatobiliary Institute, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing 210008, China.
Science Bulletin
|March 15, 2024
Summary
This study introduces a novel 3D-imprinted cell-sheet strategy for creating accurate, biomimetic hepatic microtissues. This approach enhances liver tissue engineering for disease treatment and regeneration.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Tissue Engineering
Background:
- Liver tissue engineering is crucial for treating liver diseases but faces challenges in achieving high-fidelity tissue construction.
- Accurate control over cell type, density, and distribution is essential for functional hepatic microtissues.
Purpose of the Study:
- To develop a novel three-dimensional (3D)-imprinted cell-sheet strategy for synchronous construction of biomimetic hepatic microtissues.
- To create vascularized hepatic microtissues for drug screening and liver regeneration applications.
Main Methods:
- Determined hepatic cell composition using spatial proteogenomics data.
- Utilized a homemade 3D-imprinted device for layer-by-layer imprinting and assembly of hepatic cell sheets.
- Infiltrated vascular endothelial cells into hollow sections to create vascularized channels.
Main Results:
- Successfully generated biomimetic hepatic microtissues with hexagonal hollow cross-sections and accurate cell distribution.
- Created vascularized microtissues capable of nutrient diffusion and drug perfusion.
- Demonstrated integration of microtissues into a liver-on-a-chip device and assembly into larger structures for regeneration.
Conclusions:
- The 3D-imprinted cell-sheet strategy enables high-accuracy construction of biomimetic hepatic microtissues.
- Vascularized hepatic microtissues show potential for drug screening and promoting liver regeneration.
- This strategy offers new possibilities for advanced biomimetic microtissue engineering.
