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Novel electrochemical platform based on C3N4-graphene composite for the detection of neuron-specific enolase as a biomarker for lung cancer

  • 0Cancer Research Institute, Henan Integrative Medicine Hospital 45000, Zhengzhou, China.
Scientific Reports +

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March 16, 2024

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March 16, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study presents a novel electrochemical sensor for detecting neuron-specific enolase (NSE), a biomarker for small cell lung cancer (SCLC). The graphene-g-C3N4 nanocomposite platform enables ultrasensitive and accurate NSE detection for early SCLC diagnosis.

Area Of Science

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background

  • Small cell lung cancer (SCLC) is a leading cause of cancer mortality with poor survival rates.
  • Neuron-specific enolase (NSE) is a crucial biomarker for early SCLC diagnosis and treatment monitoring.
  • Existing diagnostic methods require improvement in sensitivity and accuracy.

Purpose Of The Study

  • To develop a highly sensitive electrochemical immunosensing platform for NSE detection.
  • To utilize a novel graphene-g-C3N4 nanocomposite for enhanced electrochemical performance.
  • To validate the platform's efficacy for early SCLC diagnosis using real serum samples.

Main Methods

  • Synthesis of graphene and g-C3N4 nanosheets via liquid exfoliation.
  • Formation of a graphene-g-C3N4 nanocomposite through self-assembly.
  • Modification of screen-printed carbon electrodes with the nanocomposite and anti-NSE antibodies.
  • Electrochemical detection of NSE using impedance spectroscopy or voltammetry.

Main Results

  • The developed immunosensor demonstrated a wide linear detection range (10 pg/mL to 100 ng/mL) for NSE.
  • Achieved a low limit of detection of 3 pg/mL for NSE.
  • Exhibited excellent selectivity and accuracy in real serum samples, confirming clinical applicability.

Conclusions

  • The graphene-g-C3N4 nanocomposite-based electrochemical immunoassay offers a promising tool for ultrasensitive NSE detection.
  • This platform holds significant potential for the early diagnosis and therapeutic monitoring of SCLC.
  • The study highlights the synergistic benefits of combining graphene and g-C3N4 for advanced biosensing applications.