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Published on: September 20, 2024
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Lactiplantibacillus plantarum K8 lysates regulate hypoxia-induced gene expression
Jaehyeon Jeong1, Byeong-Hee Kang2, Sangmin Ju2
1Department of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Scientific Reports
|March 16, 2024
Summary
Lactiplantibacillus plantarum K8 (K8) lysates suppress hypoxia-induced gene expression by downregulating HIF1α accumulation. This suggests K8 may offer a novel therapeutic approach for pathologies linked to cellular hypoxia.
Area of Science:
- Cellular biology
- Microbiology
- Molecular biology
Background:
- Hypoxic responses are central to inflammation, immunity, and cancer.
- Lactiplantibacillus plantarum K8 (K8) exhibits anti-inflammatory and immunomodulatory properties.
- The impact of K8 on cellular hypoxia remains unexplored.
Purpose of the Study:
- To investigate the effect of K8 on cellular hypoxic responses.
- To determine if K8 influences hypoxia-inducible factor 1-alpha (HIF1α) signaling.
Main Methods:
- Treatment of human bone marrow and lung cancer cell lines (SH-SY5Y, H460) with K8 lysates.
- Assessment of HIF1α accumulation and target gene expression (p21, GLUT1, ALDOC) under hypoxic conditions.
- Analysis of PHD2 and VHL protein levels.
Main Results:
- K8 lysates significantly suppressed hypoxia-induced gene expression.
- K8 downregulated hypoxia-induced HIF1α accumulation in tested cell lines.
- K8 lysates and lipoteichoic acids suppressed HIF1α target gene transcription.
- K8 decreased PHD2 and VHL protein expression, suggesting a non-canonical pathway.
Conclusions:
- K8 lysates desensitize cells to hypoxic stress.
- K8 suppresses HIF1α-mediated gene activation, offering potential for therapeutic intervention in hypoxia-related diseases.
Keywords:
Lactiplantibacillus plantarum K8HIF1αHypoxic gene expressionNatural hypoxic response suppressorTranscriptional regulationMore Related Videos
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