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Antimicrobial resistance markers distribution in Staphylococcus aureus from Nsukka, Nigeria

Martina C Agbo1, Ifeoma M Ezeonu2, Beatrice O Onodagu3

  • 1Department of Pharmaceutical Microbiology and Biotechnology, University of Nigeria, Nsukka, Nigeria. martina.agbo@unn.edu.ng.

BMC Infectious Diseases
|March 16, 2024
PubMed
Abstract

Insights

Multidrug-resistant Staphylococcus aureus strains in Nigeria carry significant resistance markers like Mec A and Van A. This highlights the urgent need for surveillance and policy interventions to combat antimicrobial resistance.

Area of Science:

  • Microbiology
  • Genetics
  • Public Health

Background:

  • Multidrug resistance in Staphylococcus aureus poses global treatment challenges.
  • Genetic resistance markers contribute to the spread of multidrug-resistant S. aureus (MDR-SA).
  • This study investigated resistance marker distribution in Nigerian clinical S. aureus isolates.

Purpose of the Study:

  • To determine the prevalence and distribution of antibiotic resistance genes in clinical S. aureus isolates from Nsukka, Nigeria.
  • To characterize the antibiotic resistance profiles of these isolates.
  • To inform public health strategies for managing MDR-SA.

Main Methods:

  • Conventional culture techniques and PCR were used for S. aureus identification.
  • Disk-diffusion assays determined antibiotic resistance profiles.
  • PCR screened for specific resistance genes: Mec A, Erm A, Erm B, Erm C, Van A, and Van B.

Main Results:

  • 71.43% of isolates were confirmed as S. aureus by PCR.
  • High resistance rates were observed for oxacillin (95.72%), erythromycin (81.63%), and ertapenem (78.57%).
  • Prevalent resistance genes included Mec A (32.14%), Van A (21.43%), and Erm C (17.86%).

Conclusions:

  • The study confirms the presence of key antibiotic resistance markers in Nigerian S. aureus isolates.
  • Findings underscore the necessity for rational drug use and enhanced surveillance.
  • Effective preventive and curative policies are crucial for combating MDR-SA.
Keywords:
S. AureusAntibiotic resistanceGenesMarkers

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