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Updated: Jun 30, 2025
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Machine learning based endothelial cell image analysis of patients undergoing descemet membrane endothelial
Emine Esra Karaca1, Feyza Dicle Işık1, Reza Hassanpour2
1Department of Ophthalmology, 536164 University of Health Sciences, Ankara Bilkent City Hospital , Ankara, Türkiye.
Objectives:
In this study, we developed a machine learning approach for postoperative corneal endothelial cell images of patients who underwent Descemet's membrane keratoplasty (DMEK).
Methods:
An AlexNet model is proposed and validated throughout the study for endothelial cell segmentation and cell location determination. The 506 images of postoperative corneal endothelial cells were analyzed. Endothelial cell detection, segmentation, and determining of its polygonal structure were identified. The proposed model is based on the training of an R-CNN to locate endothelial cells. Next, by determining the ridges separating adjacent cells, the density and hexagonality rates of DMEK patients are calculated.
Results:
The proposed method reached accuracy and F1 score rates of 86.15 % and 0.857, respectively, which indicates that it can reliably replace the manual detection of cells in vivo confocal microscopy (IVCM). The AUC score of 0.764 from the proposed segmentation method suggests a satisfactory outcome.
Conclusions:
A model focused on segmenting endothelial cells can be employed to assess the health of the endothelium in DMEK patients.
