New dockside eDNA based protocol to detect the seaweed Asparagopsis armata evaluated by stakeholders

Area of Science:

• Marine biology
• Environmental science
• Biotechnology

Background:

• Ports are critical interfaces for biological invasions, necessitating rapid detection methods for invasive species.
• Early detection of invasive species like Asparagopsis armata is vital for effective management of marine ecosystems.

Purpose of the Study:

• To develop and validate a simplified, rapid end-time PCR methodology for detecting the invasive seaweed Asparagopsis armata using environmental DNA (eDNA).
• To assess the practical applicability and stakeholder acceptance of this eDNA-based detection tool in international ports.

Main Methods:

• Development of a simplified end-time PCR assay for Asparagopsis armata detection from water samples.
• Field testing of the methodology dockside in four Spanish international ports.
• Collection of feedback from port stakeholders regarding the tool's usability and potential adoption.

Main Results:

• The developed PCR method provided results within four hours of water collection.
• Asparagopsis armata was detected in three out of the four surveyed Spanish ports.
• Stakeholders unanimously approved the methodology, despite biological invasions not being their primary concern.

Conclusions:

• The simplified eDNA PCR method offers a rapid and effective tool for early detection of invasive species in port environments.
• Stakeholder feedback highlights the need for further development, including multi-species detection capabilities, for broader adoption in invasive species control.
• The study demonstrates the potential of eDNA technology for real-world application in maritime biosecurity and invasive species management.