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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jun 30, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Published on: May 23, 2025

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CRISPR activation screens: navigating technologies and applications.

Teleri Clark1, Matthew A Waller1, Lipin Loo1

  • 1Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.

Trends in Biotechnology
|March 16, 2024
PubMed
Summary
This summary is machine-generated.

CRISPR activation (CRISPRa) enables high-throughput genetic screens to identify genes that cause specific phenotypes upon upregulation. This review covers CRISPRa technologies and applications in various research fields.

Keywords:
CRISPR screensCRISPRagene activationgenetic screening

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR activation (CRISPRa) is a key tool in molecular biology.
  • CRISPRa enables high-throughput genetic screens to identify genes responsible for specific phenotypes.
  • Ongoing development aims to enhance CRISPRa efficiency and consistency.

Purpose of the Study:

  • To provide a technological overview of CRISPRa architectures.
  • To summarize pooled CRISPRa screening methodologies.
  • To discuss current and emerging applications of CRISPRa screening in research.

Main Methods:

  • Review of CRISPRa technologies.
  • Summary of pooled CRISPRa screening strategies.
  • Analysis of contemporary research applications.

Main Results:

  • Overview of available CRISPRa systems.
  • Comprehensive summary of pooled CRISPRa screens.
  • Discussion of diverse research applications.

Conclusions:

  • CRISPRa screening is a powerful approach for gene discovery.
  • The technology offers broad applicability across scientific disciplines.
  • Emerging applications promise further advancements in biological research.