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CircMCTP2 enhances the progression of bladder cancer by regulating the miR-99a-5p/FZD8 axis
Yan Liu1, Kexin Zhang2, Xianxu Yang2
1Department of Urinary Surgery, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning Province, 121000, China. liuyanforest@jzmu.edu.cn.
Journal of the Egyptian National Cancer Institute
|March 18, 2024
Summary
Circular RNA MCTP2 (circMCTP2) promotes bladder cancer progression by regulating the miR-99a-5p/FZD8/Wnt-b-catenin axis. Targeting circMCTP2 offers a potential therapeutic strategy for bladder cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Circular RNAs (CircRNAs) and microRNAs (miRNAs) play roles in tumor progression.
- CircMCTP2 is identified as a novel tumor promoter, but its function in bladder cancer remains unclear.
Purpose of the Study:
- To investigate the role and underlying mechanisms of circMCTP2 in bladder cancer development.
Main Methods:
- RT-qPCR to quantify circMCTP2 levels in bladder cancer specimens and cell lines.
- Western blotting to assess FZD8 expression.
- Cell proliferation, migration, invasion, and apoptosis assays (CCK8, Transwell, flow cytometry).
- Dual luciferase reporter assay to confirm interactions between miR-99a-5p and circMCTP2/FZD8.
Main Results:
- CircMCTP2 levels were elevated in bladder cancer, correlating with poorer survival.
- Downregulating circMCTP2 reduced cell growth and metastasis while increasing apoptosis.
- miR-99a-5p levels increased upon circMCTP2 downregulation, showing an inverse correlation in specimens.
- FZD8 was identified as a target of miR-99a-5p, and the miR-99a-5p/FZD8/Wnt-b-catenin axis mediated tumor-suppressive effects.
- CircMCTP2 promotes bladder cancer via the miR-99a-5p/FZD8/Wnt-b-catenin pathway.
Conclusions:
- CircMCTP2 acts as an oncogenic factor in bladder cancer by modulating the miR-99a-5p/FZD8/Wnt-b-catenin axis.
- This pathway regulates bladder cancer cell behaviors, highlighting circMCTP2 as a potential therapeutic target.
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