Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Autonomous biomedical research with an artificial intelligence agent.

Science (New York, N.Y.)·2026

Same author

Tumor suppressor genotype influences the extent and mode of immunosurveillance in lung cancer.

Nature communications·2026

Same author

The FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycle.

eLife·2026

Same author

A Roadmap to Transform Lung Cancer Outcomes: Priorities in Biology, Therapeutic Innovation, Early Detection, Prevention, and Interception.

Cancer discovery·2026

Same author

Metastasis as a Multiorgan Disease: Toward Organ-Informed Precision Oncology.

Cancer discovery·2026

Same author

Inactivation of CDKN2AARF Promotes p53-Independent Remodeling of the PDAC Tumor Microenvironment.

Cancer research·2026

Search research articles

Related Experiment Video

Updated: Jun 30, 2025

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

Efficient and multiplexed somatic genome editing with Cas12a mice.

Jess D Hebert, Haiqing Xu, Yuning J Tang

Biorxiv : the Preprint Server for Biology

|March 18, 2024

Summary

Researchers developed new mouse models for somatic genome editing using enhanced Acidaminococcus sp. Cas12a (enAsCas12a). This enables the creation of complex genetic changes in vivo, accelerating disease modeling and genetic interaction studies.

More Related Videos

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Related Experiment Videos

Last Updated: Jun 30, 2025

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Genetics and Genomics
Molecular Biology
Disease Modeling

Background:

Somatic genome editing in mouse models advances understanding of in vivo genetic alterations.
Current models have limitations in creating multiple targeted edits, hindering the study of complex genetic interactions.
Understanding complex genetic interactions is crucial for development, homeostasis, and disease research.

Purpose of the Study:

To accelerate and expand the generation of complex genotypes in somatic cells.
To develop novel transgenic mouse models for efficient somatic genome editing.
To facilitate high-throughput investigation of coincident genomic alterations in vivo.

Main Methods:

Generated transgenic mice expressing enhanced Acidaminococcus sp. Cas12a (enAsCas12a) under Cre-regulated and constitutive control.
Utilized Cas12a with crRNA arrays containing tandem guides for multiplex gene targeting.
Integrated modular crRNA arrays with clonal barcoding to quantify tumor formation and crRNA efficiency.

Main Results:

enAsCas12a-mediated somatic genome editing robustly generated compound genotypes in vivo.
Demonstrated the initiation of diverse cancer types driven by homozygous inactivation of tumor suppressor gene trios.
Successfully quantified tumor size, number, and crRNA efficiency using clonal barcoding.

Conclusions:

The developed Cas12a mouse alleles enable rapid generation of complex genotypes in somatic cells.
These models significantly facilitate high-throughput investigation of coincident genomic alterations in vivo.
The study provides a powerful tool for advancing research in neuroscience, cancer biology, and beyond.