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Related Concept Videos

UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Versatile open-source fluorescence documentation system.

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Updated: Jun 27, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
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Corrigendum to "Versatile open-source fluorescence documentation system" [HardwareX 15, September (2023), e00450].

Leonhard Bandilla1

  • 1Pfahlweg 18A, 82346 Andechs, Germany.

Hardwarex
|March 18, 2024
PubMed
Summary
This summary is machine-generated.

This study corrects a previously published article DOI. The correction ensures accurate citation and retrieval of scientific information for researchers.

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Area of Science:

  • Bibliometrics
  • Scientific Publishing

Context:

  • Accurate citation is crucial for scientific integrity.
  • Digital Object Identifiers (DOIs) are essential for article retrieval.

Purpose:

  • To correct the Digital Object Identifier (DOI) of a published article.
  • To ensure proper referencing and accessibility of scientific literature.

Summary:

  • The article DOI 10.1016/j.ohx.2023.e00450 has been corrected.
  • This ensures the article can be accurately located and cited by the scientific community.

Impact:

  • Improves the reliability of scientific records.
  • Facilitates seamless access to research for scholars and institutions.