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Shedding light on ONOO- detection: the emergence of a fast-response fluorescent probe for biological systems
Guoliang Wu1, Zihong Li1, Ping Huang1
1Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P. R. China. weiyinglin2013@163.com.
A new probe, NRho, detects peroxynitrite (ONOO-) for identifying tumors and inflammation. This fluorescence imaging tool offers rapid, sensitive detection in cells, tissues, and live organs, aiding biomedical research and diagnosis.
Area of Science:
- Biomedical imaging
- Molecular probes
- Reactive nitrogen species detection
Background:
- Peroxynitrite (ONOO-) is crucial in inflammation and tumor progression.
- Elevated ONOO- levels correlate with tumor advancement.
- Sensitive detection of ONOO- is vital for understanding these processes.
Purpose of the Study:
- To design and synthesize a novel rhodamine-based fluorescent probe (NRho) for ONOO- detection.
- To evaluate NRho's efficacy in identifying tumors and inflammatory sites.
- To explore variations in ONOO- levels across different cancer cell types.
Main Methods:
- Synthesis of the rhodamine-based probe NRho.
- Fluorescence imaging for ONOO- detection in cancer cells, tissues, and live organs.
- Comparative analysis of ONOO- levels in various cell lines and tissue samples.
Main Results:
- NRho was successfully synthesized with simple procedures and favorable spectral properties.
- Distinct ONOO- levels were observed in different cancer cells; hepatocellular carcinoma showed higher levels.
- NRho enabled rapid (5-minute incubation) and sensitive detection of tumors in live organs and inflammation in tissues.
Conclusions:
- The NRho probe is effective for sensitive, selective, and rapid in situ detection of ONOO-.
- NRho facilitates the identification of tumors and inflammatory tissues, with potential for clinical diagnosis.
- The probe provides new insights into ONOO- level variations in cancer and aids biomedical research.
