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Mesoporous Silica Nanoparticles-Enhanced Microarray Technology for Highly Sensitive Simultaneous Detection of
Timpika Hormsombut1,2, Nutsara Mekjinda2,3, Surachate Kalasin1
1Faculty of Science and Nanoscience & Nanotechnology Graduate Program, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
ACS Applied Bio Materials
|March 18, 2024
Summary
A novel antibody-aptamer array enables rapid, simultaneous detection of seven common foodborne pathogens. This sensitive and specific method enhances food safety by identifying bacteria like Escherichia coli and Salmonella in various samples.
Area of Science:
- Food Science
- Biotechnology
- Analytical Chemistry
Background:
- Food safety is critical for public health, necessitating accurate detection of foodborne pathogens.
- Existing methods for pathogen detection can be time-consuming and lack multiplexing capabilities.
- Developing rapid, sensitive, and specific diagnostic tools is essential for the food industry.
Purpose of the Study:
- To develop and validate a novel antibody-aptamer array for the simultaneous detection of seven prevalent foodborne pathogens.
- To enhance fluorescence signals for improved sensitivity and accuracy in pathogen detection.
- To assess the platform's performance in real food samples.
Main Methods:
- Utilized antibody-aptamer arrays for pathogen detection.
- Employed mesoporous silica nanoparticles (MSNs) conjugated with fluorescein, streptavidin, and detection antibodies to amplify fluorescence signals.
- Designed a multiplex array (7FP-biochip) for simultaneous detection of *Escherichia coli*, *Salmonella* spp., *Listeria* spp., *Shigella* spp., *Campylobacter* spp., *Clostridium* spp., and *Vibrio* spp.
Main Results:
- Achieved high sensitivity with detection limits of 10^2 CFU/mL (visual) and 20-34 CFU/g (fluorescent quantification).
- Demonstrated no cross-reactivity among the tested bacterial pathogens, ensuring high specificity.
- Successfully detected spiked pathogens in real food samples (canned tuna), validating the platform's practical application.
Conclusions:
- The developed antibody-aptamer array provides a sensitive, specific, and multiplexed platform for detecting multiple foodborne pathogens simultaneously.
- This technology offers significant potential for improving food safety monitoring and protecting public health.
- The method's advantages include reduced nonspecific binding and efficient signal enhancement.
