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Updated: Jun 30, 2025
03:22
Author Spotlight: Advancing Pathogen Detection and Disease Assessment in Real-Time Using M-ROSE
Published on: March 1, 2024
430
Microbiological Rapid On-Site Evaluation for Pulmonary Infectious Diseases
Xiangwen Weng1, Weiting Sun2, Zhenchun Luo1
1Department of Emergency and the Intensive Care Unit, Chongqing Hospital of Traditional Chinese Medicine.
Journal of Visualized Experiments : Jove
|March 18, 2024
Summary
Rapid on-site evaluation (ROSE) of microbial samples during bronchoscopy aids in quick pathogen identification for unexplained pulmonary infections. This approach enables targeted therapy, reducing broad-spectrum antibiotic use and combating antimicrobial resistance.
Area of Science:
- Pulmonary Medicine
- Infectious Diseases
- Microbiology
Background:
- Prompt empirical anti-infective therapy is vital for unexplained pulmonary infections.
- Current diagnostic methods lack specificity, requiring time-consuming tests.
- Empirical treatments can lead to antibiotic misuse and resistance.
Purpose of the Study:
- To highlight the utility of rapid on-site evaluation (ROSE) in diagnosing pulmonary infections.
- To demonstrate how ROSE facilitates timely and targeted anti-infective strategies.
- To emphasize the role of ROSE in reducing antibiotic misuse.
Main Methods:
- Utilizing bronchoscopy to obtain samples (e.g., alveolar lavage, mucosal brushing).
- Performing rapid staining and interpretation of samples during on-site evaluation (ROSE).
- Integrating microbial background information into clinical decision-making.
Main Results:
- ROSE provides essential, real-time microbial background information.
- Facilitates prompt identification of causative pathogens.
- Enables development of individualized drug therapy plans.
Conclusions:
- A standardized M-ROSE workflow improves diagnostic accuracy for pulmonary infections.
- Real-time pathogen identification supports targeted treatment and reduces broad-spectrum antibiotic use.
- This approach has significant clinical implications for patient management and antibiotic stewardship.
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