Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Absorption Spectroscopy: Lab

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Association Between Serum Vitamin D and Lipid Levels in Patients With Depression: A Cross-Sectional Study.

Alpha psychiatry·2026

Same author

Frequent Detection of Abasic Sites and <i>O</i><sup>6</sup>-Methyl-2'-Deoxyguanosine and Absence of Substituted Aromatic DNA Adducts in Colorectal Tissue of Smokers and Meat-Eaters with Colorectal Cancer.

Chemical research in toxicology·2026

Same author

A metal ion-dependent mechanism promoting gain of function in NEIL1 variants.

International journal of radiation biology·2026

Same author

Servant leadership and volunteer work engagement in blood donation services: the serial mediating roles of mission valence and volunteer motivation with the moderating role of team atmosphere.

Frontiers in psychology·2026

Same author

Engineering a Bi-BiOI Schottky Junction Rich in Oxygen Vacancies in PLLA Scaffolds to Amplify Photoelectricity-Catalysis Coupling for Accelerating Infectious Nerve Repair.

ACS applied materials & interfaces·2026

Same author

Editorial: Deep learning for high-dimensional sense, non-linear signal processing and intelligent diagnosis, vol II.

Frontiers in psychiatry·2026

Search research articles

Related Experiment Video

Updated: Jun 30, 2025

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

Mass Spectrometry-Based Method to Measure Aflatoxin B₁ DNA Adducts in Formalin-Fixed Paraffin-Embedded Tissues.

Medjda Bellamri, Lihua Yao, Rachana Tomar¹

¹Department of Chemistry, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee 37235, United States.

Chemical Research in Toxicology

|March 18, 2024

Summary

Aflatoxin B1 (AFB1) DNA adducts, AFB1-FapyGua, are stable in formalin-fixed tissues. This breakthrough allows biomonitoring of AFB1 exposure in human liver samples, aiding research on this potent carcinogen.

More Related Videos

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Published on: April 19, 2024

Related Experiment Videos

Last Updated: Jun 30, 2025

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Published on: April 19, 2024

Area of Science:

Toxicology
Molecular Biology
Cancer Research

Background:

Aflatoxin B1 (AFB1) is a potent human liver carcinogen produced by molds, contaminating staple foods.
AFB1 bioactivation forms DNA adducts, including AFB1-N7-guanine (AFB1-N7-Gua) and ring-opened AFB1-FapyGua adducts.
AFB1-FapyGua adducts are implicated in AFB1-induced G → T mutations and liver cancer, but their detection in humans is limited by tissue availability.

Purpose of the Study:

To assess the stability of AFB1-FapyGua adducts in formalin-fixed paraffin-embedded (FFPE) tissues.
To establish a method for detecting AFB1-FapyGua adducts in FFPE human liver specimens for biomonitoring.
To overcome challenges in nucleic acid quality associated with formalin fixation for accurate adduct quantification.

Main Methods:

Exposure of newborn mice to AFB1.
Analysis of AFB1-FapyGua adducts in fresh frozen and FFPE liver tissues using ion trap and Orbitrap mass spectrometry.
Development of DNA de-cross-linking retrieval processes for FFPE samples.

Main Results:

Ring-opened AFB1-FapyGua adducts are stable during formalin fixation and DNA de-cross-linking.
AFB1-FapyGua adducts in FFPE liver were detected at levels comparable to fresh frozen liver.
Orbitrap MS2 achieved a quantification limit of 4.0 adducts per 10^8 bases with 0.8 μg DNA.

Conclusions:

AFB1-FapyGua adducts are reliably detectable in FFPE liver tissues.
This DNA retrieval technology enables biomonitoring of AFB1 exposure in human cohorts at risk.
The findings facilitate research on AFB1-related liver carcinogenesis using accessible FFPE samples.