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Updated: Jun 30, 2025

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
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Multifunction fluorescence open source in vivo/in vitro imaging system (openIVIS).

John M Branning1,2, Kealy A Faughnan3, Austin A Tomson4

  • 1Quantitative Biosciences and Engineering, Colorado School of Mines, Golden, Colorado, United States of America.

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|March 18, 2024
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Summary
This summary is machine-generated.

Researchers developed a low-cost, open-source fluorescence imaging system (openIVIS) using Raspberry Pi and 3D printing. This versatile instrument enables diverse scientific measurements from lab assays to field applications, demonstrating the power of accessible technology in research and education.

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Area of Science:

  • Biophotonics
  • Open-source hardware and software
  • Low-cost scientific instrumentation

Background:

  • Open-source microcontrollers and 3D printing enable accessible scientific tools.
  • Existing low-cost instruments include microscopes, spectrometers, and sensors.
  • These devices support research, education, and experimentation across scientific disciplines.

Purpose of the Study:

  • To design and construct a multifunction fluorescent open-source in vivo/in vitro imaging system (openIVIS).
  • To integrate a Raspberry Pi, commercial cameras, LEDs, and 3D printed components.
  • To enable fluorescent measurements across the visible light spectrum with three excitation wavelengths.

Main Methods:

  • Integrated a Raspberry Pi with commercial cameras and LEDs.
  • Utilized 3D printed structures and an acrylic housing.
  • Developed Python control software for fluorescence measurements.

Main Results:

  • The openIVIS system demonstrated performance across diverse experiments, including lab assays and fieldable applications.
  • Successfully imaged plants, minerals, and tracked small animal development over ten days.
  • Measured a wide dynamic range of fluorescent responses (millimolar to picomolar) across visible wavelengths.

Conclusions:

  • Open-source hardware and software, combined with customizable manufacturing, can create versatile, low-cost scientific instruments.
  • The openIVIS system offers a flexible and powerful tool for both research and educational settings.
  • This study presents a model for developing accessible scientific instrumentation for broader scientific exploration.