Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 30, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

611

Development of a Pseudocellular System to Quantify Specific Interactions Determining the G-Quadruplex Function in

Hisae Tateishi-Karimata1, Keiko Kawauchi2, Shuntaro Takahashi1

  • 1Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.

Journal of the American Chemical Society
|March 18, 2024
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exclusion of RNA-binding domains from G-quadruplex condensates by G-quadruplex ligands.

RSC chemical biology·2026
Same author

Loss of p53 Provokes NF-κB-Dependent Disruption of Nucleolar Cap and Nucleoplasmic Redistribution of Fibrillarin During Nucleolar Stress.

Biomolecules·2026
Same author

Stability of non-canonical nucleic acid structure as a potential modulator of cell fate.

Nucleic acids research·2026
Same author

Dynamic regulation of nucleic acid replication beyond the Watson-Crick base-pairing rule.

Chemical communications (Cambridge, England)·2026
Same author

In vivo mechano-tissue engineering by hydrogels capable of transmitting intercellular mechanical stress.

Nature communications·2025
Same author

Phase Separation of RX Repeat Peptides with Nucleic Acids.

Chemistry, an Asian journal·2025

A new system called SHELL allows quantitative analysis of intracellular environments. SHELL reveals G-quadruplexes are more stable in cancer cells and K+ ions buffer against destabilization, regulating gene expression.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Intracellular chemical microenvironments regulate crucial cell behaviors like proliferation and gene expression.
  • Analyzing these complex intracellular environments quantitatively has been a significant challenge.
  • G-quadruplex formation is implicated in cancer pathogenesis.

Purpose of the Study:

  • To develop a novel system for quantitative analysis of intracellular environments.
  • To investigate the stability and behavior of G-quadruplexes within a controlled cellular environment.
  • To understand the role of ion concentrations, specifically K+, in G-quadruplex formation and gene expression regulation in cancer.

Main Methods:

  • Development of the System for Highlighting the Environment Inside of the Cell (SHELL), a pseudocellular system that removes small molecules while maintaining molecular crowding.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.3K

Related Experiment Videos

Last Updated: Jun 30, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

611
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.3K
  • Quantitative biochemical analysis of G-quadruplex formation and stability within SHELL.
  • Comparison of G-quadruplex stability under varying K+ concentrations in SHELL versus in vitro conditions.
  • Analysis of G-quadruplex-induced transcriptional inhibition patterns in SHELL derived from different cancer cell types.

    • Main Results:

    • SHELL enables precise quantitative biochemical analysis of specific factors within a controlled cellular environment.
    • G-quadruplexes exhibit greater stability in SHELL compared to in vitro conditions.
    • SHELL environments buffer against G-quadruplex destabilization at lower K+ concentrations, with the most pronounced effect observed in SHELL from nonaggressive cancer cells.
    • G-quadruplex-K+ binding stabilizes G-quadruplexes in various cancer cells, and G-quadruplex-induced transcriptional inhibition in SHELL mirrors patterns seen in living cells across different cancer stages.

    Conclusions:

    • The SHELL system provides a powerful tool for studying intracellular environments and their impact on molecular events like G-quadruplex formation.
    • Ion binding, particularly K+, plays a critical role in G-quadruplex stability and function within the cellular context of cancer.
    • G-quadruplex-mediated regulation of gene expression is a key factor in cancer pathogenesis, influenced by intracellular ion concentrations.