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KIF5B-mediated internalization of FMDV promotes virus infection

Wei Zhang1, Fan Yang1, Yang Yang1

  • 1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China; Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou 730046, China.

Virologica Sinica
|March 18, 2024
PubMed

Insights

Kinesin family member 5B (KIF5B) is crucial for foot-and-mouth disease virus (FMDV) entry into cells. This discovery offers a potential new target for developing antiviral drugs against FMDV.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Foot-and-mouth disease (FMD) is a highly contagious viral disease with significant economic impact.
  • The precise mechanism of FMD virus (FMDV) cell entry remains incompletely understood, despite identification of its cellular receptor.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying FMDV internalization into host cells.
  • To identify host factors involved in FMDV infection and replication.

Main Methods:

  • Co-immunoprecipitation (Co-IP) to confirm protein interactions.
  • Co-localization studies in infected cells.
  • KIF5B overexpression, knockdown, and knockout experiments.
  • Analysis of FMDV replication and viral particle transport.

Main Results:

  • Kinesin family member 5B (KIF5B) was identified as a key host factor in FMDV internalization.
  • Direct interaction between KIF5B and FMDV structural protein VP1 was confirmed, specifically involving the KIF5B stalk domain.
  • KIF5B overexpression enhanced FMDV replication, while KIF5B depletion suppressed it.
  • KIF5B regulates clathrin uncoating and facilitates viral particle transport to endosomes, promoting FMDV entry.

Conclusions:

  • KIF5B plays a critical role in FMDV internalization by regulating clathrin uncoating and intracellular transport.
  • The interaction between KIF5B and FMDV VP1 is essential for efficient viral entry and replication.
  • KIF5B represents a potential novel therapeutic target for FMDV antiviral drug development.
Keywords:
ClathrinEndosomeFMDVKIF5BVP1 protein

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