Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Urinary kallikrein: assay validation and physiological variability.

K O Ash, J B Smith, M Lynch

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |September 30, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ubiquitination of protein kinase C-alpha and degradation by the proteasome.

    The Journal of biological chemistry·1996
    Same author

    The glucocorticoid attenuated response genes GARG-16, GARG-39, and GARG-49/IRG2 encode inducible proteins containing multiple tetratricopeptide repeat domains.

    Archives of biochemistry and biophysics·1996
    Same author

    Extracellular ATP increases cytosolic calcium in cultured rat renal arterial smooth muscle cells.

    Clinical and experimental pharmacology & physiology·1996
    Same author

    Calcium bioavailability from bovine milk and dairy products in premenopausal women using intrinsic and extrinsic labeling techniques.

    The Journal of nutrition·1996
    Same author

    Pyoderma gangrenosum in a patient with cryoglobulinemia and hepatitis C successfully treated with interferon alfa.

    Journal of the American Academy of Dermatology·1996
    Same author

    Cutaneous manifestations and consequences of smoking.

    Journal of the American Academy of Dermatology·1996

    Urinary kallikrein (UK) levels are a potential preclinical indicator for hypertension. This study validated UK assays and found daily variations, with higher morning excretion, but no significant impact from common lifestyle factors.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Nephrology

    Background:

    • Decreased urinary kallikrein (UK) output is a potential preclinical indicator of essential hypertension.
    • Understanding UK assay parameters and physiological variability is crucial for hypertension research.

    Purpose of the Study:

    • To assess selected urinary kallikrein assay parameters.
    • To evaluate the physiological variability of urinary kallikrein output.
    • To prepare for future UK studies in hypertension-prone Utah kindreds.

    Main Methods:

    • Colorimetric kallikrein assay validation, including precision (CV < 5% within-run, 14% day-to-day), recovery (105%), and correlation with 3H-TAME esterase method (r=0.990).
    • Assessment of urine specimen stability at room temperature and various frozen conditions (-20°C, -80°C).

    Related Experiment Videos

  • Analysis of diurnal UK output patterns and intra-/inter-individual variations (mean 20%).

    • Main Results:

    • The colorimetric UK assay demonstrated acceptable precision and stability.
    • Urinary kallikrein output exhibited significant diurnal variation, peaking in the morning.
    • Intra- and inter-individual variations in UK output were comparable (mean 20%).
    • UK output increased with age in children, reaching adult levels by age 15; male and female values were similar.
    • Lifestyle factors (smoking, alcohol, coffee, etc.) and female hormone medications did not significantly affect 12-hour UK output in 1110 subjects.

    Conclusions:

    • The validated colorimetric assay is suitable for UK studies.
    • Significant diurnal variation in UK output necessitates standardized collection times.
    • UK output shows developmental trends and is largely unaffected by common lifestyle factors in the studied population.