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Functionalized magnetic lipase/Cu3(PO4)2 hybrid nanoflower: Synthesis, characterization, and enzymatic evaluation
Shamini Anboo1, Sie Yon Lau1, Jibrail Kansedo1
1Department of Chemical Engineering, Faculty of Engineering and Science, Curtin University Malaysia, CDT 250, 98009, Miri, Sarawak, Malaysia.
Heliyon
|March 19, 2024
Summary
We synthesized magnetic lipase/Cu3(PO4)2 hybrid nanoflowers using ultrasonication. These magnetic biocatalysts show enhanced activity and reusability, making them efficient for industrial applications.
Area of Science:
- Biocatalysis
- Materials Science
- Nanotechnology
Background:
- Enzyme immobilization is crucial for industrial biocatalysis.
- Developing efficient and reusable biocatalysts remains a challenge.
- Magnetic nanoparticles offer advantages in catalyst recovery and reuse.
Purpose of the Study:
- To synthesize magnetic lipase/Cu3(PO4)2 hybrid nanoflowers.
- To investigate the immobilization mechanism and factors affecting nanoflower morphology.
- To evaluate the catalytic activity, stability, and reusability of the hybrid nanoflowers.
Main Methods:
- Rapid ultrasonication method for synthesis.
- Characterization of nanoflower morphology and magnetic properties.
- Assay of enzyme immobilization yield and catalytic activity under varying conditions.
Main Results:
- Successfully synthesized magnetic lipase/Cu3(PO4)2 hybrid nanoflowers.
- Optimized immobilization conditions (temperature, pH, duration).
- Achieved a catalytic enhancement of 89% compared to free lipase, with 60% activity retained after 5 cycles.
Conclusions:
- Magnetic lipase/Cu3(PO4)2 hybrid nanoflowers are highly efficient biocatalysts.
- The strong magnetic property facilitates easy recovery and reuse.
- These nanoflowers show significant potential for industrial biocatalytic applications.
