A Novel DNBS-based Fluorescent Probe for the Detection of H₂S in Cells and on Test Strips

Hydrogen sulfide (H₂S) plays a key role in the physiology and pathology of organisms, and H₂S in the environment is easily absorbed and harmful to health. It is of great significance to develop a probe with good selectivity, high sensitivity and good stability that can detect hydrogen sulfide inside and outside organisms. In this work, we designed a novel "turn-on" fluorescent probe CIM-SDB for the detection of H₂S. The probe CIM-SDB used indene-carbazole as the fluorophore and 2,4-dinitrobenzenesulfonyl as the recognition site. The probe CIM-SDB exhibited high selectivity and sensitivity to H₂S (detection limit as low as 123 nM). Moreover, the probe CIM-SDB was successfully applied to the detection of intracellular exogenous and endogenous H₂S, and the test strips prepared by the probe CIM-SDB could realize the convenient and rapid detection of H₂S.