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Immunophenotyping of Leukocytes in Amniotic Fluid
Yi Xu1, Derek Miller2, Jose Galaz3
1Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2024
Abstract:
Immunophenotyping allows for the deep characterization of leukocytes present in biological samples. Here, we describe a complete procedure for the immunophenotyping of amniotic fluid, which can provide information into the immune processes taking place in the amniotic cavity. The protocol describes amniotic fluid cell count determination, processing, and the use of viability, extracellular antibody, and intracellular/intranuclear antibody staining prior to flow cytometer acquisition.

