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Related Experiment Video

Updated: Jun 30, 2025

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

smFISH for Plants.

Sahar Hani^1,2, Caroline Mercier^1,3, Pascale David¹

¹Aix Marseille Univ, CEA, CNRS, BIAM, UMR7265, Saint-Paul lez Durance, France.

Methods in Molecular Biology (Clifton, N.J.)

|March 19, 2024

Summary

We present a single-molecule fluorescence in situ hybridization (smFISH) protocol optimized for plants. This method enables visualization and quantification of RNA molecules, revealing gene expression and RNA dynamics at the single-molecule level.

Keywords:

Imaging Plant RNA Single-moleculeSingle molecule Transcription smFISH

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Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Area of Science:

Molecular Biology
Plant Science
Genetics

Background:

Single-molecule fluorescence in situ hybridization (smFISH) is crucial for visualizing and quantifying RNA at the single-molecule level.
Its application in plants is limited due to specific hybridization and imaging challenges.
Understanding gene expression and RNA dynamics in plants requires advanced molecular techniques.

Purpose of the Study:

To develop and present an optimized smFISH experimental workflow for plant research.
To overcome existing challenges in applying smFISH techniques to plant tissues.
To enable high-resolution analysis of gene expression and RNA dynamics in plants.

Main Methods:

Detailed protocol for sample preparation in plants.
Optimized probe hybridization strategies for plant tissues.
Advanced signal detection techniques for single RNA visualization.

Main Results:

Successfully adapted smFISH for plant samples, overcoming previous limitations.
Demonstrated visualization and quantification of individual RNA molecules in intact plant cells.
Established a robust workflow for analyzing gene expression at the single-molecule level in plants.

Conclusions:

The presented smFISH protocol significantly enhances the study of gene expression in plants.
This technique provides novel insights into RNA dynamics and cellular heterogeneity in whole plants.
The workflow holds great promise for advancing plant molecular biology research.