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Updated: Jul 8, 2026

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Published on: January 21, 2016
Detection of membrane fission in single Bacillus subtilis cells during endospore formation with high temporal
Ane Landajuela1, Martha Braun2, Christopher D A Rodrigues3
1Cellular and Molecular Physiology, Yale University, New Haven, CT, USA; Nanobiology Institute, Yale University, West Haven, CT, USA.
Abstract:
Membrane fission is an essential process in all domains of life. The underlying mechanisms remain poorly understood in bacteria, partly because suitable assays are lacking. Here, we describe an assay to detect membrane fission during endospore formation in single Bacillus subtilis cells with a temporal resolution of ∼1 min. Other cellular processes can be quantified and temporally aligned to the membrane fission event in individual cells, revealing correlations and causal relationships. For complete details on the use and execution of this protocol, please refer to Landajuela et al.1.
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