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Updated: Jun 30, 2025

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness
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FOXQ1 inhibits the progression of osteoarthritis by regulating pyroptosis

Zhihuan Luo1, Hui Zeng1, Kanghua Yang1

  • 1Department of Sports Medicine, Ganzhou People’s Hospital, Ganzhou 341000, Jiangxi Province, China.

Aging
|March 19, 2024
PubMed
Abstract

Insights

Forkhead box Q1 (FOXQ1) inhibits osteoarthritis progression by suppressing NLRP3-induced pyroptosis. This study reveals FOXQ1 as a potential therapeutic target for managing this common age-related joint disease.

Area of Science:

  • Biomedical research
  • Molecular biology
  • Genetics

Background:

  • Osteoarthritis (OA) is a prevalent age-related joint disease.
  • NLRP3-induced pyroptosis is implicated in OA pathogenesis.
  • The regulatory mechanisms of NLRP3 and pyroptosis in OA are not fully understood.

Purpose of the Study:

  • To investigate the role of FOXQ1 in osteoarthritis.
  • To elucidate the mechanism by which FOXQ1 affects chondrocyte pyroptosis and OA progression.

Main Methods:

  • Transcriptome sequencing in an OA mouse model.
  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • In vitro cell models (IL-1β-induced ATDC5 cells) to assess cell proliferation (CCK-8 assay), apoptosis (flow cytometry), protein expression (Western blot), and inflammatory responses (ELISA).

Main Results:

  • FOXQ1 was found to be downregulated in the OA mouse model.
  • Overexpression of FOXQ1 promoted chondrocyte proliferation and inhibited apoptosis.
  • FOXQ1 suppressed key pyroptosis-related proteins (NLRP3, Caspase-1, GSDMD) and inflammatory cytokines (IL-6, IL-18, TNF-α).

Conclusions:

  • FOXQ1 plays an inhibitory role in osteoarthritis progression.
  • The mechanism involves the downregulation of NLRP3-induced pyroptosis.
  • FOXQ1 represents a potential therapeutic target for osteoarthritis.
Keywords:
FOXQ1inflammasomeosteoarthritispyroptosis

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