A perovskite-based electrochemiluminescence aptasensor for tetracycline screening

Area of Science:

• Analytical Chemistry
• Materials Science
• Biotechnology

Background:

• Antibiotic residues, particularly tetracyclines, pose significant risks to public health and food safety.
• Developing sensitive and reliable detection methods for these residues is crucial.

Purpose of the Study:

• To develop a novel electrochemiluminescence (ECL) sensing strategy for the quantitative detection and differentiation of tetracycline antibiotics.
• To enhance the stability and biocompatibility of perovskite quantum dots for biosensing applications.

Main Methods:

• Surface modification of perovskite with cetyltrimethylammonium bromide to create stable ECL emitters.
• Development of a perovskite quantum dot-based ECL aptasensor.
• Utilizing aptamer-triggered strain displacement reactions to disrupt an ECL quenching system (perovskite and silver nanoclusters).
• Quantitative detection of tetracycline, chlortetracycline, and oxytetracycline.

Main Results:

• The modified perovskite exhibited stable ECL emission in aqueous systems.
• The aptasensor demonstrated high sensitivity and selectivity for target tetracyclines.
• Successful differentiation and quantification of three common tetracycline antibiotics.
• Achieved a linear range of 0.1-10 μM for oxytetracycline with good recovery rates (94.7%-101.6%) in real samples.

Conclusions:

• The developed perovskite quantum dot-based ECL aptasensor is a sensitive and practical tool for detecting antibiotic residues.
• This method holds potential for routine monitoring of tetracycline contamination in food and environmental samples.
• The strategy offers a promising advancement in biosensing for public health safety applications.