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Site-specific albumin tagging with NIR-II fluorogenic dye for high-performance and super-stable bioimaging
Ningning Zhu1,2, Jiajun Xu1,2, Qi Su1,2
1Joint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, First Hospital of Jilin University, Jilin University, Changchun 130021, P.R. China.
Theranostics
|March 20, 2024
Summary
Researchers developed novel near-infrared-II (NIR-II) dyes for specific albumin targeting in deep tissue imaging. This creates brighter, more stable probes for enhanced angiography and lymphography with improved pharmacokinetics.
Area of Science:
- Biomedical Imaging
- Organic Chemistry
- Molecular Biology
Background:
- Synthetic near-infrared-II (NIR-II) dyes show potential for deep tissue imaging but lack specificity for biomolecules.
- The interaction mechanism between albumin and cyanine molecules is poorly understood, often forming uncertain complexes.
Purpose of the Study:
- To develop NIR-II fluorogenic dyes for site-specific albumin tagging.
- To investigate the interaction mechanism between NIR-II fluorogenic dyes and albumin.
- To create high-performance cyanine@albumin probes for bioimaging.
Main Methods:
- Design and synthesis of novel NIR-II fluorogenic dyes.
- Site-specific labeling of albumin with the developed dyes.
- Proteomic analysis and docking modeling to identify binding sites.
- Evaluation of probe performance in NIR-II angiography and lymphography.
Main Results:
- Identified CO-1080 as an optimal dye structure for stable and bright NIR-II cyanine@albumin probes.
- CO-1080 demonstrated maximum binding affinity to albumin and catalyzed covalent attachment.
- Precise binding sites on albumin (Cys476 and Cys101) were identified.
- The cyanine@albumin probe exhibited improved pharmacokinetics compared to the free dye.
Conclusions:
- Developed a novel cyanine@albumin probe for high-performance NIR-II angiography and lymphography.
- Site-specific labeling occurs under mild conditions, offering a straightforward synthesis strategy.
- The probes are suitable for targeting bioimaging and imaging-guided surgery.
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