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Updated: May 1, 2026
Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Video-rate endocavity photoacoustic/harmonic ultrasound imaging with miniaturized light delivery
Donghyeon Oh1, Hyunhee Kim1, Minsik Sung1
1Pohang University of Science and Technology, Medical Device Innovation Center, Departments of Electrical Engineering, Convergence IT Engineering, Mechanical Engineering, Medical Science and Engineering, Pohang, Republic of Korea.
Significance:
Endocavity ultrasound (US) imaging is a frequently employed diagnostic technique in gynecology and urology for the assessment of male and female genital diseases that present challenges for conventional transabdominal imaging. The integration of photoacoustic (PA) imaging with clinical US imaging has displayed promising outcomes in clinical research. Nonetheless, its application has been constrained due to size limitations, restricting it to spatially confined locations such as vaginal or rectal canals.
Aim:
This study presents the development of a video-rate (20 Hz) endocavity PA/harmonic US imaging (EPAUSI) system.
Approach:
The approach incorporates a commercially available endocavity US probe with a miniaturized laser delivery unit, comprised of a single large-core fiber and a line beamshaping engineered diffuser. The system facilitates real-time image display and subsequent processing, including angular energy density correction and spectral unmixing, in offline mode.
Results:
The spatial resolutions of the concurrently acquired PA and harmonic US images were measured at and in the radial direction, respectively, and 1.22 deg and 1.50 deg in the angular direction, respectively. Furthermore, the system demonstrated its capability in multispectral PA imaging by successfully distinguishing two clinical dyes in a tissue-mimicking phantom. Its rapid temporal resolution enabled the capture of kinetic dye perfusion into an ex vivo porcine ovary through the depth of porcine uterine tissue. EPAUSI proved its clinical viability by detecting pulsating hemodynamics in the male rat's prostate in vivo and accurately classifying human blood vessels into arteries and veins based on measurements.
Conclusions:
Our proposed EPAUSI system holds the potential to unveil previously overlooked indicators of vascular alterations in genital cancers or endometriosis, addressing pressing requirements in the fields of gynecology and urology.
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