Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Summer‑time performance of urban tree and shrub species as particulate matter biofilters: a case study on species‑level tolerance and PM accumulation in Malda, Eastern India.

International journal of phytoremediation·2026

Same author

A quantitative framework to assess the potential of earlier cancer detection to improve cancer survival.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026

Same author

Modeling pulsed magneto-optical stimulation for controlled nanoparticle aggregation and hyperthermia in a non-Newtonain aqueous medium adjacent to a tumor.

Computers in biology and medicine·2026

Same author

Corrigendum to "The cellular consequences of particulate matter pollutants in plants: Safeguarding the harmonious integration of structure and function" [Sci. Total Environ. 914 (2024), 169763].

The Science of the total environment·2026

Same author

DeBERTa-BiLSTM: a multi-label classification model for depression emotions.

Cognitive neurodynamics·2026

Same author

Bifurcation analysis and fear-induced reactions in non-refuged prey with cooperative hunting among predators: Deterministic and stochastic dynamics.

Mathematical biosciences·2026

Same journal

Cortical Brain Entropy Architecture Reveals Multidimensional Structure of Schizophrenia.

Biophysical reports·2026

Same journal

The oligomeric state of chitooligosaccharide deacetylase from the marine bacterium Vibrio campbellii.

Biophysical reports·2026

Same journal

Quantifying Species-Specific Binding Affinities of Transthyretin Aggregation Inhibitors.

Biophysical reports·2026

Same journal

Drosophila jump muscle myofibrils: A new tool for investigating activation and relaxation.

Biophysical reports·2026

Same journal

Optical tweezers combined with FRET tension sensor reveal force-dependent vinculin dynamics.

Biophysical reports·2026

Same journal

Role of E. coli acid resistance systems in proton motive force formation during fermentation.

Biophysical reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 30, 2025

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Video-microscopy-based automated trajectory determination.

Christopher Tyson^1,2, Santosh Gaire^1,3, Ian Pegg^1,3

¹Vitreous State Laboratory, Washington, District of Columbia.

Biophysical Reports

|March 20, 2024

Summary

This summary is machine-generated.

This study introduces a novel tracking method for numerous, fast, and indistinguishable particles in complex force fields. The algorithm accurately determines object trajectories using only centroid data, even in challenging, dense conditions.

More Related Videos

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Related Experiment Videos

Last Updated: Jun 30, 2025

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Area of Science:

Physics
Materials Science
Biotechnology
Engineering

Background:

Tracking indistinguishable objects in dense, high-velocity scenarios presents significant challenges for existing algorithms.
Current methods struggle with high object densities and rapid movements, especially within force fields.

Purpose of the Study:

To develop a robust tracking algorithm for densely clustered, high-velocity, indistinguishable objects.
To enable accurate trajectory determination using only object centroids, minimizing reliance on other image information.

Main Methods:

A novel algorithm employing a scoring function and backtracking refinement process.
Minimal restrictions on object velocities and accelerations.
Utilizes only object centroids as input for tracking.

Main Results:

Successful tracking of hundreds of particles in challenging environments.
Maintains accuracy even when object displacement approaches inter-object separation.
Demonstrated capability in simulated and experimental data (superparamagnetic microspheres).

Conclusions:

The developed method offers a robust solution for tracking challenging particle dynamics.
Its wide applicability in video microscopy spans biology, materials science, physics, and engineering.
Addresses limitations of existing tracking methods in dense, dynamic systems.