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Updated: Jun 30, 2025
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Published on: December 4, 2017
Data-Independent Acquisition and Label-Free Quantification for Quantitative Proteomics Analysis of Human
Karl A T Makepeace1, Alexander W Rookyard1, Lipi Das1
1Department of Biological Sciences, Quantitative Proteomics and Metabolomics Center, Columbia University, New York, New York.
Abstract:
This article presents a practical guide to mass spectrometry-based data-independent acquisition and label-free quantification for proteomics analysis applied to cerebrospinal fluid, offering a robust and scalable approach to probing the proteomic composition of the central nervous system. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Cerebrospinal fluid sample collection and preparation for mass spectrometry analysis Basic Protocol 2: Mass spectrometry sample analysis with data-independent acquisition Support Protocol: Data-dependent mass spectrometry and spectral library construction Basic Protocol 3: Analysis of mass spectrometry data.
