Exocyclic DNA adducts and oxidative stress parameters: useful tools for biomonitoring exposure to aldehydes in
Héléna Alamil1,2, Marie-Lise Colsoul3, Natacha Heutte4
1Normandie University, UNICAEN, ABTE EA4651, Caen, France.
Summary
This study explored aldehyde-induced DNA adducts in smokers, finding correlations with antioxidant levels. These findings may lead to new biomarkers for aldehyde exposure and cancer risk.
Area of Science:
- Biomarkers and Oxidative Stress
- Environmental Health and Toxicology
Background:
- Exocyclic DNA adducts are potential biomarkers for cancer risk linked to oxidative stress and aldehyde exposure in smokers.
- Aldehydes can originate from tobacco combustion or endogenous lipid peroxidation.
Purpose of the Study:
- To investigate the relationship between nine aldehyde-induced DNA adducts and antioxidant activities.
- To evaluate novel biomarkers for systemic aldehyde exposure.
Main Methods:
- Quantified adduct levels in blood DNA of 34 active smokers using UPLC-MS/MS.
- Measured levels of antioxidant vitamins (A, C, E), coenzyme Q10, β-carotene, superoxide dismutase (SOD), and autoantibodies against oxidized LDL.
Main Results:
- Aldehyde-induced adducts reflected oxidative production from lipid peroxidation.
- Significant correlation found between SOD and crotonaldehyde adducts (p=0.0251).
- β-Carotene negatively correlated with formaldehyde (p=0.0351) and acetaldehyde adducts (p=0.0413); Vitamin C showed an inverse trend with acetaldehyde adducts (p=0.0584).
Conclusions:
- The study identified promising correlations between aldehyde DNA adducts and antioxidant levels in smokers.
- Further research on a larger cohort is underway to assess the impact of smoking cessation on adduct profiles and antioxidant activities.
